Laboratory study: 10 Lumbricus terrestris were kept in closed boxes (32x25x10 cm = 8 litres) containing 4 litres of uncontaminated natural soil.
Lysosomal membrane stability: The nutral red retention time (NRR-T) assay. pH 5.4. The test containers with soil (750 g of Fox sand) and 10 worms were randomly placed into prepared holes in outside plots, where they would be exposed to natural climatic conditions. Soil exposure experiment: Two metal-contaminated field soil. Experiment 2: Soil from following depth 70-90, 90-140, 140-300, 300-500, and 500-700 cm. Rearing experiments. © Copyright 2020 CAB International. Cu concentration (for CuSO4) 0, 250, 500 and 750 Cu ha-1. Generate a print friendly version containing only the sections you need. The time taken by earthworms to burrow entirely into the soil (15 mm depth) was recorded at 1, 3, 5, 10, 15, 20, 25 and 30 min. Reynolds, J. W.; Wetzel, M. J. Reynolds, John Warren, and Mark Julian Wetzel, 2008: Terrestrial Oligochaeta (Annelida: Clitellata) in North America, including Mexico, Puerto Rico, Hawaii, and Bermuda. Pedozoologica Hungarica. 1, 278pp. Lethality test (14 days-LC50): Ten worms were placed in glass containers with 500 g dry mass of a soil-like substrate composed of a mixture of sphagnum peat, kaolinite clay, industrial quartz sand in a dry-weight ratio of 1:2:7.
Replicates (n=3) for each species at each site and each concentration of sodium arsenate were stored in the dark at 9 degrees C. Arsenate toxicity: The uncontaminated soil was treated with sodium arsenate heptahydrate (494 mg As kg-1). Megadrilogica, vol. The mine strain was obtained from Shipham, UK. Lumbricus rubellus is meestal roodbruin tot roodpaars gekleurd, irriserend aan de rugzijde en bleek-geel aan de buikzijde. Pornainen (less Pb contaminated situated control soil) about 25 km to the north-east of Tikkurila. 9. vi + 33. Experiment 3: Arsenate doses 40 ug/g dry weight. Soil discrimination: Soil was placed in Petri dishes with no divider separating the two soils. New Brunswick Museum Monographic Series (Natural Science), no. Sampling time is day 4, 7, 14 and 21. Field-collected adults: Specimens of mature. This site has been subject to aerial deposition of cadmium, copper, lead, and zinc since 1929. with one half containing the uncontaminated soil and the other containing the same soil treated with 0, 12, 25, 123, 247, 494 or 1235 mg As kg-1 as sodium arsenate, with a divider separating the two soils. RANK 2 = a combination of the sublethal endpoints. The bags were sealed and kept in the dark at 9 degrees C. Six replicates. Earthworm (n=3) were introduced to each side of the dish. (2018). LC50 using Probit analysis on SPSS statistical software version 10.01. Megadrilogica, vol. Field experiment: Test containers were made with flexible corrugated drainage tiles with vinyl fly screens fixed over both ends. 11. Along the pollution gradient, as species richness decreased, Lumbricus castaneus became more dominant. During exposure, sample earthworms were removed at 1, 4, 8, 16, 32, 64 and 120 hr. 8-10 animals were used in each experimental group. In the middle of the sampled area of each plot, about 500 earthworms, obtained from a vermiculturist, were introduced. Earthworms (n=3) introduced onto the soil in each side of the dish. Worm: Eighty earthworms were added to 12 kg of the treated Kettering loam. http://creativecommons.org/licenses/by-nc-nd/4.0/, http://creativecommons.org/licenses/by-nc/4.0/, http://creativecommons.org/licenses/by-sa/4.0/, http://creativecommons.org/licenses/by/4.0/.
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